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Watch the Full Video at https://www.jove.com/v/52984/engineer.... Engineered Vascularized Muscle Flap - a 2 minute Preview of the Experimental Protocol Dana Egozi, Yulia Shandalov, Alina Freiman, Dekel Rosenfeld, David Ben-Shimol, Shulamit Levenberg Kaplan Medical Center, Department of Plastic Surgery; Technion Israel Institute of Technology, Biomedical Engineering; Technion Israel Institute of Technology, Interdepartmental Program in Biotechnology; Technion Israel Institute of Technology, Medicine Department; To date, thick tissue defects are typically reconstructed by applying autologous tissue flaps or engineered tissues. In this protocol, we present a new method for engineering vascularized tissue flap bearing an autologous pedicle, to serve as a substitute to autologous flaps. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments