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Watch the Full Video at https://www.jove.com/v/54554/experime.... Experimental Infection with Listeria monocytogenes as a Model for Studying Host Interferon-γ Responses - a 2 minute Preview of the Experimental Protocol Jeeyoon Jennifer Ahn, Thirumahal Selvanantham, Monan Angela Zhang, Thierry Mallevaey, Shannon E. Dunn University of Toronto, Department of Immunology; University Health Network, Toronto General Research Institute; Women's College Research Institute,; This protocol describes how to inoculate C57BL/6J mice with the EGD strain of Listeria monocytogenes (L. monocytogenes) and to measure interferon-γ (IFN-γ) responses by natural killer (NK) cells, natural killer T (NKT) cells, and adaptive T lymphocytes post-infection. This protocol also describes how to conduct survival studies in mice after infection with a modified LD50 dose of the pathogen. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments