Perivascular Precursor Cell populations isolated from Cardiac tissue | Protocol Preview скачать в хорошем качестве

Perivascular Precursor Cell populations isolated from Cardiac tissue | Protocol Preview

Watch the Full Video at https://www.jove.com/v/54252/isolatio.... Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue - a 2 minute Preview of the Experimental Protocol James E. Baily, William C.W. Chen, Nusrat Khan, Iain R. Murray, Zaniah N. González Galofre, Johnny Huard, Bruno Péault University of Edinburgh, Centre for Cardiovascular Science, Queen's Medical Research Institute; University of Pittsburgh, Department of Bioengineering and Orthopaedic Surgery; Massachusetts Institute of Technology, Research Laboratory of Electronics and Department of Biological Engineering; University of Edinburgh, MRC Centre for Regenerative Medicine; University of Pittsburgh, Stem Cell Research Center, Department of Orthopaedic Surgery; University of Texas Health Science Center at Houston, Department of Orthopaedic Surgery; University of California at Los Angeles, Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine; Human cardiac tissue harbours multipotent perivascular precursor cell populations that may be suitable for myocardial regeneration. The technique described here allows for the simultaneous isolation and purification of two multipotent stromal cell populations associated with native blood vessels, i.e. CD146+CD34- pericytes and CD34+CD146- adventitial cells, from the human myocardium. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments