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Hey my fellow EM aficionados! A while back, I did a demo covering STEM-EELS elemental mapping using our Themis S/TEM equipped with a GIF Continuum ER Energy Filter and Gatan DigitalMicrograph (please see the link below). • FEI Themis Z S/TEM + Gatan Continuum ER en... In that video, I used the "AutoFocus" function in DigitalMicrograph to focus the ZLP in the EEL spectrum, which is works by automatically adjusting a series of focusing elements to minimize the FWHM of the ZLP in the EEL spectrum (ideally collected from vacuum). While this is generally effective and very easy to do, my experience is that this usually doesn't produce the best possible result. To obtain the best possible focusing of the ZLP, this needs to be done manually, but fear not, my fellow EM aficionados; this is far less daunting than it may seem and often only requires noticeable tweaking of "FX" (focus X) and "FY" (focus Y) for the spectrometer. For added benefit, I often find that manual focusing is much faster than the "AutoFocus" function, too. Just one very important point: DO NOT enable ZLP lock during manual tuning! If you do this and the ZLP is substantially defocused (either by accident or intentionally), ZLP lock will hang up and cause DigitalMicrograph to crash; only enable ZLP lock once focusing is complete. Thank you for your support, my fellow EM aficionados, as we have now surpassed 5,400 subscribers! Please like, subscribe, and share and leave any questions or comments you may have and I will do my best to reply as soon as possible. Video topic requests are always welcome and appreciated; I enjoy making these videos and wish I could make them more frequently, but the demands of my job make it tough to do so; I’m in charge of three S/TEMs, three dual FIB/SEM systems (a new one recently signed off on), and half of an SEM and this keeps me very busy! Connect with me on LinkedIn: / nicholas-rudawski-30414528 Where I work: https://nrf.aux.eng.ufl.edu/ E-mail me directly: [email protected]