Top 20 HPLC Troubleshooting Problems | Causes, Remedies & Real Lab Tips скачать в хорошем качестве

Top 20 HPLC Troubleshooting Problems | Causes, Remedies & Real Lab Tips

#troubleshooting #interview #hplc Practical HPLC troubleshooting for QC and R&D — 20 real problems (from high back pressure to SST failures) with lab-proven remedies you can use today. This sequel to our ‘Top 20 HPLC Interview Questions’ focuses on shop-floor fixes and step-by-step isolation tests. Top 20 HPLC Troubleshooting Problems — Causes, Remedies & Real Lab Tips ‪@PharmaHealthInsights‬ with Rajesh — Practical HPLC troubleshooting for QC, R&D and analysts. 🎯 WATCH NEXT: Top 20 HPLC Interview Questions (my previous video) ▶︎    • Top 20 HPLC Interview Questions | What Int...   🔔 SUBSCRIBE for weekly pharma & lab tutorials:    / @pharmahealthinsights   ------------------------- CHAPTERS / TIMESTAMPS 00:06 Intro — Why troubleshooting matters 00:40 Problem 1 — High Back Pressure 02:10 Problem 2 — Low Back Pressure 03:05 Problem 3 — No Peaks Observed 03:55 Problem 4 — Split Peaks 04:40 Problem 5 — Tailing Peaks 05:25 Problem 6 — Fronting Peaks 06:12 Problem 7 — Baseline Drift 07:09 Problem 8 — Noisy Baseline 08:05 Problem 9 — Ghost Peaks 09:10 Problem 10 — Retention Time Shift 10:03 Problem 11 — Broad Peaks 11:03 Problem 12 — Pump Not Delivering Flow 11:55 Problem 13 — Pressure Fluctuation 12:43 Problem 14 — Carryover 13:35 Problem 15 — Peak Area Variation 14:23 Problem 16 — Negative Peaks 15:12 Problem 17 — Column Deterioration 16:02 Problem 18 — Detector Saturation 16:52 Problem 19 — Software / Integration Errors 17:40 Problem 20 — System Suitability Failure 18:45 Closing & key takeaways ------------------------- KEY TAKEAWAYS: • Follow a stepwise approach: isolate, verify, fix (remove column → check system). • Prevention beats cure: filter/degass solvents, maintain column care SOP, and run system suitability every day. • This video is the practical follow-up to our Top 20 HPLC Interview Questions — link above. 📚 Useful links and references: — More videos and quick tips playlist:    • Pharma Essentials: Complete Knowledge Hub ...   👍 LIKE, SHARE & SUBSCRIBE if this helped — comment below which HPLC problem you face most often. Get all Pharma and Healthcare content and tips at: Linkedin: @mahealth-insights/ Facebook:@9733 Twitter: @9733 Blog: https://rajeshkapoorpharma.blogspot.com WhatsApp: @0029VavmGBn05MUb71JrZY2f Mini website: https://deltacard.me/pharmahealthinsi... #chromatography #analyticalchemistry #PharmaQC #PharmaRND #pharmatraining #LabTroubleshooting #qualitycontrol #pharmacareers #pharmaeducation #GMPTraining #pharmaindustry