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Batch Normalization for Dummies

Worried that having multiple batches of patient samples will inject technical variance into your cytometry dataset? This YouTube video will highlight the value of batch normalization for reducing technical noise—also known as batch effects—that can arise from operator handling differences, machine-to-machine variability, or site-to-site variability. These challenges are especially common in extended clinical trials, where patient samples arrive at lab sites on a rolling basis. We also give a light introduction to how common normalization algorithms, such as CytoNorm or CyCombine, normalize the cytometry datasets. 00:00 Introduction 00:28 Who Cares? 00:43 Issues with Batching Samples 01:06 Batch Effects 02:35 Goal of Batch Normalization 03:17 Increasing Batch Comparisons 04:28 Batch Normalization Algorithms #Cytometry #ClinicalTrials #LifeSciences #BatchNormalization #DataAnalysis #Immunotherapy #TeikoBio