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Watch the Full Video at https://www.jove.com/v/3138/in-vivo-l.... In vivo Liver Endocytosis Followed by Purification of Liver Cells by Liver Perfusion - a 2 minute Preview of the Experimental Protocol Sandhya Gopalakrishnan, Edward N. Harris University of Nebraska, Lincoln, Department of Biochemistry; The study of liver sinusoidal endothelial cells (SECs) must be performed with primary cells obtained from the animal as no cell lines exist. This method relies on liver digestion and differential centrifugation for SEC purification for subsequent culturing and experimentation. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments