Generation: Carcinoma-Associated Fibroblasts From Human Mammary Fibroblasts l Protocol Preview скачать в хорошем качестве

Generation: Carcinoma-Associated Fibroblasts From Human Mammary Fibroblasts l Protocol Preview

Watch the Full Video at https://www.jove.com/v/3201/experimen.... Experimental Generation of Carcinoma-Associated Fibroblasts (CAFs) from Human Mammary Fibroblasts - a 2 minute Preview of the Experimental Protocol Urszula M. Polanska, Ahmet Acar, Akira Orimo University of Manchester, CR-UK Stromal-Tumour Interaction Group, Paterson Institute for Cancer Research; Juntendo University, Atopy Research Center; Carcinoma-associated fibroblasts (CAFs) rich in myofibroblasts present within the tumour stroma, play a major role in driving tumour progression. We developed a coimplantation tumour xengraft model for experimentally generating CAFs from human mammary fibroblasts. The protocol describes how to establish CAF myofibroblasts that acquire an ability to promote tumourigenesis. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments