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Watch the Full Video at https://www.jove.com/v/55120/rapid-is.... Rapid Isolation of BMPR-IB+ Adipose-Derived Stromal Cells for Use in a Calvarial Defect Healing Model - a 2 minute Preview of the Experimental Protocol Clement D. Marshall, Elizabeth R. Zielins, Elizabeth A. Brett, Charles P. Blackshear, Michael S. Hu, Tripp Leavitt, Leandra A. Barnes, H. Peter Lorenz, Michael T. Longaker, Derrick C. Wan Stanford University School of Medicine, Hagey Laboratory for Pediatric Regenerative Medicine, Division of Plastic and Reconstructive Surgery, Department of Surgery; Adipose-derived stromal cells may be useful for engineering new tissue from a patient's own cells. We present a protocol for the isolation of a subpopulation of human adipose-derived stromal cells (ASCs) with increased osteogenic potential, followed by application of the cells in an in vivo calvarial healing assay. Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments