Non-Laser Capture Microscopy : Microdissection-Discrete Mouse Brain Regions l Protocol Preview скачать в хорошем качестве

Non-Laser Capture Microscopy : Microdissection-Discrete Mouse Brain Regions l Protocol Preview

Watch the Full Video at https://www.jove.com/v/3125/non-laser.... Non-Laser Capture Microscopy Approach for the Microdissection of Discrete Mouse Brain Regions for Total RNA Isolation and Downstream Next-Generation Sequencing and Gene Expression Profiling - a 2 minute Preview of the Experimental Protocol Norman Atkins, Charlie M. Miller, Joseph R. Owens, Fred W. Turek Northwestern University, Center for Sleep and Circadian Biology; RNA expression profiling of discrete mouse brain regions requires a precise and repeatable tissue collection strategy. A protocol that uses both coronal brain sectioning and tissue corer-assisted microdissection is described here. The yield and quality of total RNA obtained from the resulting samples confirms the utility of the outlined method. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments