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Watch the Full Video at https://www.jove.com/v/62882/isolatio.... Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests - a 2 minute Preview of the Experimental Protocol Yao-Chia Liu, Nian-Tong Ni, Ju-Chun Chang, Yi-Hsuan Li, Mi Rong Lee, Jae Su Kim, Yu-Shin Nai National Chung Hsing University, Department of Entomology; Jeonbuk National University, Department of Agricultural biology; Jeonbuk National University, Department of Agricultural Convergence Technology; Here we present a protocol based on the mealworm (Tenebrio molitor)-bait system that was used for isolating and selecting entomopathogenic fungi (EPF) from soil samples. An effective conidia number (ECN) formula is used to select high stress tolerant EPF based on physiological characteristics for pest microbial control in the field. Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments