Скачать с ютуб Weighted Gene Co-expression Network Analysis (WGCNA) Step-by-step Tutorial - Part 2 в хорошем качестве

Weighted Gene Co-expression Network Analysis (WGCNA) Step-by-step Tutorial - Part 2

This is part 2 of step-by-step tutorial of Weighted Gene Co-expression Network Analysis (WGCNA). In this video I demonstrate how to correlate modules to phenotypes/traits of interest and perform intramodular analysis by identifying hub genes using module measures and calculate gene significance. In addition, I provide an intuition about associating module eigengenes (continuous variable) to a trait of interest (categorical variable). I hope you find this video helpful! I look forward to your comments in the comment section below! Part 1 of this tutorial:    • Weighted Gene Co-expression Network A...   Data: https://www.ncbi.nlm.nih.gov/geo/quer... Code: https://github.com/kpatel427/YouTubeT...) WGCNA Tutorial: https://horvath.genetics.ucla.edu/htm... Chapters 0:00 Intro 00:03 Relate modules to phenotypes/traits 00:14 Binarize categorical variables (traits) 6:03 Correlate module eigengenes with traits 7:23 Visualize module-trait relationship as a heatmap 12:12 Extract genes from modules of interest 13:36 Does it make sense to use Pearson correlation to correlate a continous variable (module eigengene) with a categorical variable (disease severity)? 16:13 Intramodular analysis: get genes with high module membership (hub genes) 17:30 Intramodular analysis: get genes significantly associated with traits of interest You can show your support and encouragement by buying me a coffee: https://www.buymeacoffee.com/bioinfor... To get in touch: Website: https://bioinformagician.org/ Github: https://github.com/kpatel427 Email: [email protected] #bioinformagician #bioinformatics #wgcna #coexpressionnetworks #geneexpression #scalefreenetworks #proteinproteininteractionnetworks #sequencing #coverage #samtools #depthofsequencing #samflag #sam #bam #alignment #phred #fasta #fastq #singlecell #10X #ensembl #biomart #annotationdbi #annotables #affymetrix #microarray #affy #ncbi #genomics #beginners #tutorial #howto #omics #research #biology #GEO #rnaseq #ngs