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Watch the Full Video at https://www.jove.com/v/54278/reconsti.... Reconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets - a 2 minute Preview of the Experimental Protocol Mathijs Vleugel, Sophie Roth, Celebrity F. Groenendijk, Marileen Dogterom Delft University of Technology, Department of Bionanoscience; The assembly and positioning of the mitotic spindle depend on the combined forces generated by microtubule dynamics, motor proteins and cross-linkers. Here we present our recently developed methods in which the geometrical confinement of spherical emulsion droplets is used for the bottom-up reconstitution of basic mitotic spindles. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments