Functional NMJ and Motor Units Generation in Microfluidic Devices | Protocol Preview скачать в хорошем качестве

Functional NMJ and Motor Units Generation in Microfluidic Devices | Protocol Preview

Watch the Full Video at https://www.jove.com/v/62959/generati.... Generation of Human Motor Units with Functional Neuromuscular Junctions in Microfluidic Devices - a 2 minute Preview of the Experimental Protocol Katarina Stoklund Dittlau, Emily N. Krasnow, Laura Fumagalli, Tijs Vandoorne, Pieter Baatsen, Axelle Kerstens, Giorgia Giacomazzi, Benjamin Pavie, Elisabeth Rossaert, Jimmy Beckers, Maurilio Sampaolesi, Philip Van Damme, Ludo Van Den Bosch KU Leuven - University of Leuven, Department of Neurosciences, Experimental Neurology, and Leuven Brain Institute; , VIB Center for Brain & Disease Research, Laboratory of Neurobiology; , VIB Center for Brain & Disease Research, Research Group Molecular Neurobiology; KU Leuven - University of Leuven, VIB Bio Imaging Core; KU Leuven - University of Leuven, Department of Development and Regeneration, Stem Cell and Developmental Biology; University Hospitals Leuven, Department of Neurology; We describe a method to generate human motor units in commercially available microfluidic devices by co-culturing human induced pluripotent stem cell-derived motor neurons with human primary mesoangioblast-derived myotubes resulting in the formation of functionally active neuromuscular junctions. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments