Isotope Days 2025-NMR with Haribabu Arthanari, PhD скачать в хорошем качестве

Isotope Days 2025-NMR with Haribabu Arthanari, PhD

Join Dr. Haribabu Arthanari from Harvard Medical School & Dana-Farber Cancer Institute for “Using Fluorine as an Atomic Beacon for Probing Structure and Dynamics of Biomolecules”—an eye-opening look at how the unique properties of fluorine are advancing biomolecular NMR, even in today’s era of AI-driven structure prediction. As proteins get bigger, traditional NMR probes like amides and methyls start to fall short—leaving aromatic residues, the heart of many protein cores and active sites, frustratingly invisible. Dr. Arthanari’s talk dives into how fluorine, with its bio-orthogonality and remarkable sensitivity to local environment, offers a new window into both protein and RNA structure and dynamics. He explains the technical challenges that have held fluorine back in high-molecular-weight systems—especially fast relaxation due to chemical shift anisotropy (CSA)—and then walks viewers through creative solutions: TROSY effects, innovative pulse sequences, and strategic isotope labeling. See how his team combines theoretical calculations, custom-labeled amino acids, and clever biochemistry (including codon expansion and site-specific incorporation) to sharpen resolution and reveal previously hidden states in proteins like GB1, MVP, and the massive proteasome. Dr. Arthanari also demonstrates the power of 2D CF and CH correlations, not just for proteins but also for RNA, and touches on how these methods can be multiplexed for drug screening or extended to nucleic acid bases. Along the way, he shares both the “aha” moments and the real-world limitations—making this talk as honest as it is practical. Perfect for NMR enthusiasts, biomolecular researchers, and anyone curious about how fluorine labeling and advanced spectroscopy are changing what’s possible in structural biology and drug discovery. Learn why aromatic residues are finally getting the attention they deserve—and how a single atom can light up the darkest corners of molecular structure and dynamics. Check out our Biomolecular NMR page on our website: https://isotope.com/applications-bionmr