Urine and Stool (Feaces) MCS (Microscopy, culture and sensitivity) - Laboratory practicals скачать в хорошем качестве

Urine and Stool (Feaces) MCS (Microscopy, culture and sensitivity) - Laboratory practicals

Urine and Stool MCS: Laboratory Practicals Urine MCS Sample Collection Clean-catch mid-stream urine (MSU) in a sterile container Label and transport to lab promptly Microscopy 1. *Wet mount*: Examine for: Leukocytes (WBCs) Red blood cells (RBCs) Epithelial cells Bacteria Yeast Parasites (e.g., Trichomonas) 2. *Gram stain*: Identify bacteria, yeast Culture 1. *Inoculate*: Urine onto: Blood agar MacConkey agar 2. *Incubate*: 37°C, 18-24 hours 3. *Identify*: Bacterial colonies (e.g., E. coli, Klebsiella) Sensitivity 1. *Antibiotic discs*: Place on agar plate 2. *Incubate*: 37°C, 18-24 hours 3. *Measure zones*: Inhibition zones (mm) 4. *Interpret*: Susceptible, Intermediate, Resistant (SIR) Stool MCS Sample Collection Fresh stool in a clean container Label and transport to lab promptly Microscopy 1. *Direct smear*: Examine for: Parasites (e.g., ova, cysts, larvae) WBCs, RBCs 2. *Wet mount*: Examine for: Motile organisms (e.g., Giardia, Trichomonas) Culture 1. *Inoculate*: Stool onto: MacConkey agar XLD agar (for Salmonella, Shigella) TCBS agar (for Vibrio) 2. *Incubate*: 37°C, 18-24 hours 3. *Identify*: Bacterial colonies (e.g., E. coli, Salmonella) Sensitivity 1. *Antibiotic discs*: Place on agar plate 2. *Incubate*: 37°C, 18-24 hours 3. *Measure zones*: Inhibition zones (mm) 4. *Interpret*: Susceptible, Intermediate, Resistant (SIR) Safety Precautions Wear gloves, lab coat Handle samples with care (biohazard risk) Dispose of materials properly Would you like more details on interpreting results or lab procedures? #microscopy