Embedding aortic rings in a collagen matrix. скачать в хорошем качестве

Embedding aortic rings in a collagen matrix.

Materials required for embedding are shown: the 96-well plate and sterile forceps. The unpolymerised collagen mixture consisting of 10x DMEM, sterile distilled water and type 1 rat tail collagen is visible as a yellow liquid. After adding a small drop of 5N NaOH to the mixture and mixing well, the pH increases and the mixture turns pink. 50 µl aliquots of this mixture are transferred to the 96-well plate and aortic rings carefully embedded in them, with care taken to remove as much Opti-MEM® from the ring as possible so as not to dilute the collagen matrix excessively (as this would impair polymerization). Full protocol described in: Baker, M. et al. Use of the mouse aortic ring assay to study angiogenesis. Nature Protocols 7, 89--104 (2012) doi:10.1038/nprot.2011.435 http://dx.doi.org/10.1038/nprot.2011.435