Flagella Staining: Leifson Method скачать в хорошем качестве

Flagella Staining: Leifson Method

First of all take two hours old flagellated cell culture slant and add two to three drops of sterile distill water in the slant with the help of sterile pipette. Note that the distill water is added slowly without disturbing the growth of cells. After addition of distill water incubated the slant for 20 minutes. Then take a drop of suspension from the slant and place the drop on a clean slide which is kept in slanting position. The drop should flow slowly from one end of slide to other end to avoid folding of flagella on cell. Allow smear to air dry here we don’t use heat fixation treatment . After air drying the slide is flooded with Leifson’s stain till a thin film of shinny surface appear. After this give a gentle stream of water wash treatment to a slide. Now treat the slide with 1 % methylene blue treatment for 1 minute. Give the slide water wash treatment ,air dry and observe under oil immersion lens.