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Watch the Full Video at https://www.jove.com/v/3024/mouse-ute.... Mouse in Utero Electroporation: Controlled Spatiotemporal Gene Transfection - a 2 minute Preview of the Experimental Protocol Asuka Matsui, Aya C. Yoshida, Mayumi Kubota, Masaharu Ogawa, Tomomi Shimogori RIKEN Brain Science Institute, Lab for Molecular Mechanisms of Thalamus Development; A gene transfer method into the developing mouse brain is described by using a unique surgical method and special shape of electrodes. This unique technique allows transfection of plasmid DNA temporally and spatially, which will aid many neuroscientists in studying brain development. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments