Stepwise Video Guide of Gel Electrophoresis (animation) скачать в хорошем качестве

Stepwise Video Guide of Gel Electrophoresis (animation)

This is the 3rd video in the series and is on electrophoresis. The link to the other 2 videos are the following DNA extraction protocol from Bacteria:    • DNA Extraction Protocol from Bacteria Expl...   How to make PCR reaction mixture and run PCR:    • How to make PCR reaction mixture and run PCR   DNA gel electrophoresis is a powerful technique used to analyze DNA fragments. It's an essential tool for scientists who want to study the genetic material of organisms and understand their biological processes. To understand how DNA gel electrophoresis works, let's start by looking at the apparatus. It consists of a gel, typically made of agarose, which is placed in a horizontal electrophoresis chamber. Before we load the DNA sample, it must be mixed with a loading buffer. This buffer contains a tracking dye, which helps us visualize the DNA as it moves through the gel, and a density agent, which makes the sample sink to the bottom of the well. Once the DNA sample is loaded onto the gel, an electric current is applied across the chamber. The DNA fragments are negatively charged, so they move towards the positively charged electrode. As they move through the gel, the smaller fragments move faster than the larger fragments. After the electrophoresis is complete, the DNA fragments are visualized by staining the gel with a fluorescent dye. The dye binds to the DNA, creating bands that can be seen under UV light. By analyzing the size and pattern of the bands, we can determine important information about the DNA fragments. For example, we can determine the size of the fragment, and if it matches a known sequence, we can confirm the identity of the DNA. DNA gel electrophoresis is a versatile and powerful tool that has revolutionized the field of molecular biology. Its applications are vast, ranging from forensics to genetic engineering, and it continues to be a crucial technique in modern scientific research.