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Watch the Full Video at https://www.jove.com/v/57651/light-sh.... Light-sheet Microscopy for Three-dimensional Visualization of Human Immune Cells - a 2 minute Preview of the Experimental Protocol Rouven Schoppmeyer, Renping Zhao, Markus Hoth, Bin Qu Saarland University, Department of Biophysics, Center for Integrative Physiology and Molecular Medicine (CIPMM), School of Medicine; Here, we present a protocol to visualize immune cells embedded in a three-dimensional (3D) collagen matrix using light-sheet microscopy. This protocol also elaborates how to track cell migration in 3D. This protocol can be employed for other types of suspension cells in the 3D matrix. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments