Electroporation/Primary Neuronal Culture: Studying Gene Function l Protocol Preview скачать в хорошем качестве

Electroporation/Primary Neuronal Culture: Studying Gene Function l Protocol Preview

Watch the Full Video at https://www.jove.com/v/2103/in-utero-.... In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons - a 2 minute Preview of the Experimental Protocol Heather Rice, Seiyam Suth, William Cavanaugh, Jilin Bai, Tracy L. Young-Pearse Brigham and Woman's Hospital and Harvard Medical School, Center for Neurologic Diseases; University of Connecticut, Department of Physiology and Neurobiology; In utero electroporation is a valuable method for transfecting neuronal progenitor cells in vivo. Depending upon the placement of the electrodes and the developmental timepoint of electroporation, certain subsets of cortical cells can be targeted. Targeted cells can then be analyzed in vivo or in vitro for effects of genetic alteration. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments