Virus Genomes by Comparing DNA from Virion and from Infected Leaves | Protocol Preview скачать в хорошем качестве

Virus Genomes by Comparing DNA from Virion and from Infected Leaves | Protocol Preview

Watch the Full Video at https://www.jove.com/v/57855/combinin.... Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes - a 2 minute Preview of the Experimental Protocol Jeanmarie Verchot, Aastha Thapa, Dulanjani Wijayasekara, Peter R. Hoyt Texas A&M Agrilife Center at Dallas,; Oklahoma State University, Noble Research Center; The University of Tulsa, Department of Biology, College of Engineering and Natural Sciences; Oklahoma State University, Bioinformatics and Genomics Core Facility, Department of Biochemistry and Molecular Biology; Here we present a new approach to identify plant viruses with double-strand DNA genomes. We use standard methods to extract DNA and RNA from infected leaves and carry out next-generation sequencing. Bioinformatic tools assemble sequences into contigs, identify contigs representing virus genomes and assign genomes to taxonomic groups. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments