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🧬 INSIDE SEMAGLUTIDE PRODUCTION Journey inside the pharmaceutical facility producing Semaglutide, the active ingredient in weight loss and diabetes medications including Ozempic and Wegovy. From peptide synthesis to pre-filled injection devices, witness every stage of the GLP-1 receptor agonist manufacturing process that has transformed diabetes and obesity treatment worldwide. ⏱️ CHAPTERS: 00:00 - Introduction 00:08 - Facility Overview and Biosafety Protocols 00:40 - Peptide Synthesis Laboratory 01:28 - Solid-Phase Peptide Synthesis SPPS 02:48 - Amino Acid Chain Assembly 04:16 - Cleavage and Deprotection 05:36 - HPLC Purification Process 07:04 - Lyophilization Freeze-Drying 08:24 - Formulation and Compounding 09:44 - Sterile Filtration Systems 10:56 - Syringe Filling Operations 12:16 - Quality Control and Potency Testing 13:28 - Device Assembly 14:40 - Final Packaging and Cold Chain 15:52 - Distribution and Logistics 16:44 - Conclusion 📊 PRODUCTION AT SCALE: Semaglutide: GLP-1 receptor agonist peptide with 31 amino acids Used for Type 2 diabetes and chronic weight management Peptide synthesis via solid-phase methodology Purification to over 99 percent pharmaceutical purity Sterile filling in controlled environment ISO Class 5 cleanroom Pre-filled injection devices 0.25mg, 0.5mg, 1mg, 2mg doses Cold chain storage required 2-8°C throughout distribution Weekly subcutaneous injection regimen FDA approved for diabetes in 2017 and weight loss in 2021 🔬 SCIENTIFIC BACKGROUND: Semaglutide is a glucagon-like peptide-1 or GLP-1 receptor agonist used to treat type 2 diabetes and obesity. It works by mimicking the GLP-1 hormone, stimulating insulin secretion, reducing appetite, and slowing gastric emptying. The medication has shown significant efficacy in clinical trials for both glycemic control and weight reduction. Brand names include Ozempic for diabetes, Wegovy for weight management, and Rybelsus as oral form. The manufacturing process requires advanced biotechnology including solid-phase peptide synthesis, sophisticated purification techniques, and sterile pharmaceutical production under stringent regulatory standards. 💉 MANUFACTURING HIGHLIGHTS: Peptide synthesis: 31 amino acid sequence assembly Chain length: 4,113 daltons molecular weight Purification: Multi-step HPLC with over 99 percent purity requirement Formulation: pH-controlled solution with stabilizers Filling: Automated sterile filling 1.5ml or 3ml cartridges Device: Pre-filled injection system for patient use Testing: Potency, purity, sterility, endotoxin verification Storage: Refrigerated cold chain throughout distribution 🎯 ABOUT INSIDE PHARMA: This documentary series explores pharmaceutical and biotechnology manufacturing at industrial scale. We document real production processes showing how modern medications are made. Episode 1: COVID-19 mRNA Vaccine Production Episode 2: Sildenafil Tablet Production Episode 3: Semaglutide GLP-1 Weight Loss Drug - This video Episode 4: Coming soon. 🔔 SUBSCRIBE for pharmaceutical and biotech manufacturing documentaries #Semaglutide #Ozempic #Wegovy #WeightLoss #GLP1 #DiabetesMedication #PharmaceuticalManufacturing #PeptideSynthesis #Biotechnology #InsidePharma #PeptideProduction #WeightLossInjection #DiabetesTreatment #PharmaceuticalIndustry #Documentary DISCLAIMER: This video is for educational purposes documenting pharmaceutical manufacturing processes. Semaglutide, Ozempic, and Wegovy are registered trademarks of Novo Nordisk. This video is not sponsored by or affiliated with Novo Nordisk. All information presented is for educational documentation of pharmaceutical manufacturing techniques. Consult healthcare professionals regarding medical treatment decisions.