WHS Session L: WOUND HEALING FOUNDATION - 3M AWARD LECTURE скачать в хорошем качестве

WHS Session L: WOUND HEALING FOUNDATION - 3M AWARD LECTURE

Moderators: Laura Parnell, BS, MS, CWS; Joshua Tam, PhD Speaker: Veronica Haywood, DPT Diabetic Lower Extremity Ulcerations (DLEU's) are a common complication of diabetes (DM) that severely impact quality of life and can lead to amputation. With DM reaching epidemic proportions, a thorough understanding of the pathogenesis involved in impaired healing is required. Previous studies suggest that DM can alter glycosylation related gene expression leading to changes in protein sialylation and fucosylation. A disruption of the normal patterns of enzymatic protein glycosylation during wound healing would be expected to lead to functional alterations in cellular-protein interactions and signaling, thereby affecting wound healing and epithelial integrity. Although the expression and activity of ceramide glycosyl transferases are recognized as a necessary component of normal epidermal homeostasis and skin barrier function, most other glycosylation related pathways have been ignored during normal and DM cutaneous wound healing. Our preliminary studies have identified multiple glycosylation gene pathways that are differentially expressed in DM and normal skin wound healing, including polysialylation. Additionally, our analysis of previously published datasets identified alterations in N- and O- linked glycosylation related pathways in ulcerated versus intact human DM skin. With funding from the Wound Healing Society Foundation 3M Fellow Award, we are investigating the regulation of enzymatic protein glycosylation during diabetic and non-diabetic wound healing to determine whether diabetes induces impairments in skin wound healing by altering the pattern of normal protein glycosylation.