Advanced Targeted Sequencing: Unraveling the Complexity of Plant Genetic Diversity скачать в хорошем качестве

Advanced Targeted Sequencing: Unraveling the Complexity of Plant Genetic Diversity

Abstract: Access to high-quality plant genome assemblies has greatly advanced our understanding of species diversity. The significant reduction in Long Read sequencing costs has democratized access to biodiversity studies. However, exploring intra-species variability within regions of interest (ROI), especially in large genomes, remains expensive. In this context, targeted enrichment methods emerge as a strategic solution for analyzing biodiversity across broader sample sets. These approaches provide precise and reliable data, enabling the identification of genomic regions associated with key traits in specific genotypes. The French Genomic Center (CNRGV) provides three cutting-edge/innovative strategies for efficiently capturing large regions of interest in complex plant genomes, including Twist Target Enrichment. To demonstrate the potential of this approach, we conducted a pilot project to characterize a 60Kb locus involved in pathogen resistance in common bean. We then further validated the technology by applying it to a more complex plant species, bread wheat, to characterize a 750Kb region of interest. Key Takeaways: Discover how targeted enrichment methods efficiently explore intra-species variability in plant genomes. Understand the application of Twist Target Enrichment combined with Long Read sequencing for analyzing complex plant genetic diversity. Learn about the CNRGV's innovative strategies for capturing large regions of interest in plant genomes. See real-world examples of successful targeted sequencing in common bean and bread wheat. Speaker Details: Caroline Callot, Engineer, INRAE - CNRGV William Marande, Engineer, INRAE - CNRGV Speaker Bio: Caroline Callot works in the wet lab team, and William Marande is part of the bioinformatics team at INRAE-CNRGV. They collaborate with public and private partners on projects focused on the characterization of whole genomes or targeted genomic regions across various plant species.