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Watch the Full Video at https://www.jove.com/v/57789/membrane.... Membrane Remodeling of Giant Vesicles in Response to Localized Calcium Ion Gradients - a 2 minute Preview of the Experimental Protocol Baharan Ali Doosti, Ann-Sofie Cans, Gavin D.M. Jeffries, Tatsiana Lobovkina Chalmers University of Technology, Department of Chemistry and Chemical Engineering; We present a technique for contactless micromanipulation of vesicles, using localized calcium ion gradients. Microinjection of a calcium ion solution, in the vicinity of a giant lipid vesicle, is utilized to remodel the lipid membrane, resulting in the production of membrane tubular protrusions. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments