Professor Steve Hill on the characterisation of GPCR Protein Ligands using NanoBRET™ technology скачать в хорошем качестве

Professor Steve Hill on the characterisation of GPCR Protein Ligands using NanoBRET™ technology

Professor Steve Hill from the University of Nottingham talks about the characterisation of GPCR Protein Ligands using NanoBRET™ technology. NanoBRET™ technology enables the study of protein:protein interactions in real time using full-length proteins expressed at physiologically relevant levels in the cell. In this short video early adopter Professor Steve Hill from the University of Nottingham describes how NanoBRET technology was used to investigate the drug-induced modulation of ligand-binding to cell surface G protein-coupled receptor (GPCR) and receptor tyrosine kinase (RTK) proteins. By attaching a bright luminescent protein (NanoLuc®) to a GPCR or RTK, such as VEGFR2, Professor Hill’s work demonstrates the utilisation of NanoBRET in monitoring cell surface receptor-ligand interactions in real time.