Detection Protocol and Stripping the Membrane | Chemiluminescence: Detection скачать в хорошем качестве

Detection Protocol and Stripping the Membrane | Chemiluminescence: Detection

Detection Protocol and Stripping the Membrane After the secondary antibody incubation and four washes are complete, add room temperature substrate to blot and let it incubate for 3-5 minutes with gentle agitation. Check the manufacturer recommendations for exact incubation times. After incubation, move on to the detection using either film or a digital imager. Stripping is the term used to describe the removal of primary and secondary antibodies from a Western blot membrane. A single blot can be analyzed sequentially with multiple antibodies by stripping one antibody from the blot and subsequently incubating with an additional antibody. About LICORbio LICORbio offers imaging systems, analysis software, and IRDye® infrared dye reagents for quantitative protein and small animal imaging. LICORbio provides an integrated solution for quantitative Western blots with its Odyssey® Infrared Imaging Systems, Image Studio™ quantification software, Empiria Studio® analysis software for publication-ready data, reagents, unique IRDye® secondary antibodies, and online training courses. For chemiluminescent Western blots, the C-DiGit® Blot Scanner is a personal, portable imager. Use the Odyssey® XF or Odyssey M Imaging Systems for both chemiluminescence and near-infrared fluorescence Western blots. You can achieve superior small animal imaging results using BrightSite™ near-infrared fluorescent probes or bioluminescence on the Pearl® Trilogy imaging system. LICORbio Website https://www.licorbio.com/ Reagent Store https://www.licor.com/bio/reagents Image Studio™ Software https://www.licor.com/image-studio LinkedIn   / licorbio   Twitter   / licorbio   Facebook   / licorbio