Part II: Purification and Cultivation of Human Islets in Human Pancreatic Islet Isolation скачать в хорошем качестве

Part II: Purification and Cultivation of Human Islets in Human Pancreatic Islet Isolation

Reference: https://app.jove.com/v/1343/human-pan... The second part of the process involves the purification and cultivation of human islets in the isolation of human pancreatic islets. This step is crucial in ensuring the quality and viability of the islets for further research or transplantation purposes. During the purification stage, the islets are separated from other pancreatic tissues and impurities. This is done using various techniques such as density gradient centrifugation, which allows the islets to be isolated based on their specific density. By carefully layering the pancreatic tissue sample on top of a density gradient medium and subjecting it to centrifugation, the islets can be collected in a purified form. Once the purification is complete, the isolated islets are then transferred to a culture medium for cultivation. This medium provides the necessary nutrients and conditions for the islets to survive and grow. It typically contains a mixture of essential nutrients, growth factors, and hormones that support the metabolic needs of the islets. The cultivation process allows the islets to recover from the isolation procedure and regain their functionality. Cultivating human islets is a delicate process that requires careful monitoring and maintenance. The culture medium needs to be regularly replenished to ensure optimal conditions for the islets. The temperature, pH level, and oxygen supply are also closely regulated to mimic the natural environment of the pancreas. This allows the islets to maintain their insulin-producing capabilities and overall functionality.