The screw technique for conducting intracranial xenograft research in murine models скачать в хорошем качестве

The screw technique for conducting intracranial xenograft research in murine models

Reference: https://app.jove.com/t/3157/a-simple-... This guide provides a straightforward approach to utilizing the screw method for conducting intracranial xenograft studies in murine models. The screw method is a surgical technique that allows for the precise implantation of xenograft tissues into the brain of mice, facilitating the study of tumor biology and therapeutic responses in a controlled environment. This procedure is particularly valuable for researchers aiming to investigate the interactions between human tumor cells and the murine brain microenvironment, as it enables the establishment of a more relevant model for studying cancer progression and treatment efficacy. To begin, it is essential to prepare the necessary materials and equipment, including a stereotactic frame, surgical instruments, and the xenograft tissue itself. The mice should be anesthetized appropriately to ensure minimal discomfort during the procedure. Once the animal is secured in the stereotactic frame, a small incision is made in the scalp to expose the skull. Using a drill, a precise hole is created in the skull at the desired coordinates, allowing for the careful insertion of the screw or cannula that will hold the xenograft tissue. This step requires meticulous attention to detail to avoid damaging surrounding brain structures. After the xenograft tissue is implanted, the surgical site must be closed properly to promote healing and minimize the risk of infection. Post-operative care is crucial, as it involves monitoring the mice for any signs of distress or complications. Researchers should also establish a timeline for follow-up assessments, which may include imaging studies or histological analyses to evaluate tumor growth and response to treatment. By adhering to this simple yet effective screw method, researchers can enhance the reliability and relevance of their intracranial xenograft studies, ultimately contributing to advancements in cancer research and therapeutic development.