Rolling Circle Replication | Molecular Biology | Bio-Matters скачать в хорошем качестве

Rolling Circle Replication | Molecular Biology | Bio-Matters

#Molecular Biology #replication #DNA Replication #Rolling Circle Replication#biotechnology #biology #Application of Biotechnology #Biotechnology in medical sector #Application of Medical Biotechnology #application #covid19 #Bio-Matters Video Explanation: Rolling circle replication. DNA replication initiates when the Rep-A protein binds to the origin of replication or Ori, a specific section of the plasmid's double-stranded DNA. Rep-A is synthesized from a gene carried by the plasmid itself. Having bound to Ori, Rep-A introduces a single-strand break (nick) in one of the DNA strands. The Rep-A protein remains attached to the five-prime end of the nicked strand. The free three-prime end of the nicked DNA strand, now equipped with a hydroxyl group, serves as a primer for the DNA polymerase enzyme of the host cell. The host DNA polymerase utilizes this primer to initiate synthesis of the complementary strand. Rep-A recruits a helicase enzyme that facilitates the unwinding of the DNA at the replication fork. Single-strand DNA binding proteins are recruited to coat the exposed single-stranded regions, thereby stabilizing them. Single strand is rejoined by the Rep a protein and released. Then the DNA ligase seals the nick in the double-stranded molecule, the single-stranded DNA can now undergo replication, creating a looped region that allows RNA polymerase access to the DNA, forming a primer. Host DNA polymerase utilizes the primer as a starting point for DNA synthesis. Finally, DNA ligase seals any remaining nicks, resulting in the formation of a complete, double-stranded plasmid. Each of these plasmids can undergo replication through the same methods, resulting in the generation of numerous copies of the plasmid.