Selection of Ion Exchange Medias Using A Unicorn Method on the AKTA Avant. скачать в хорошем качестве

Selection of Ion Exchange Medias Using A Unicorn Method on the AKTA Avant.

This video shows how to write a method to test several ion exchange columns at once. Here I use anion exchange but the principle would work for cation exchange as well. It's a long video so get some popcorn and get ready to nerd out over chromatography. If you ever actually watch it leave a comment, I'd be grateful. For more reading on creating methods: https://cdn.cytivalifesciences.com/ap... Getting your system started for a run: https://b3p.it.helsinki.fi/download/A... Reach me at my Linkedin page   / chad-blamey-ph-d-649a336   Here is the method so you can use it yourself, no copyright or anything. 0.00 Base: CV, Vc=0.962 {ml}, (HiTrap Q FF, 1 ml)#Column 0.00 Phase: Method Settings 0.00 Base: SameAsMain 0.00 Alarm pre column pressure: Enabled, (0.50)#Pre column pressure limit {MPa}, 0.00 {MPa} 0.00 Wavelength: 280 {nm}, Off {nm}, Off {nm} 0.00 Noise reduction UV: 5.0 {sec} 0.00 Injection valve: Manual load 0.00 Outlet valve: Out-Waste 0.00 pH valve: In-line, In-line 0.00 Alarm air sensors: Enabled, Enabled, Disabled 0.00 Inlet A: (A1)#Inlet A 0.00 Inlet B: (B1)#Inlet B 0.00 Pump wash: (Off)#Wash A, (Off)#Wash B, Off, (Off)#Wash Sample Buffer Inlet 0.00 End_Block 0.00 Phase: System Flush 0.00 Base: Volume, Any 0.00 Block: Flowrate 0.00 Base: SameAsMain 0.00 System flow: (1.000)#Flow rate {ml/min}, Pre column pressure 0.00 End_Block 5.00 End_Block 0.00 Phase: Column equilibration 0.00 Base: SameAsMain 0.00 Gradient: 0.0 {%B}, 0.00 {base} 0.00 Column position: (Position 1)#Column Position, Down flow (10.00)#Column Equilibration length End_Block 0.00 Phase: Load Sample 0.00 Base: Volume, Any 0.00 System flow: 0.000 {ml/min}, Off 0.00 Injection valve: Direct inject 0.00 Auto zero UV 0.00 Sample inlet: (S1)#Sample inlet 0.00 Sample flow: (1.000)#Loading flowrate {ml/min}, Off 0.00 Injection mark 0.00 Peak fractionation parameters: Level, 0.150 {min}, (100.000)#Start Frac Threshold {mAU}, 100.000 {mAU/min}, (100.000)#End Peak Frac Threshold {mAU}, 75.000 {mAU/min} 0.00 Peak fractionation: Volume, 96 deep well plate, 2.00 {ml}, Next tube 0.00 Hold until: Air sensor Sample inlet valve, Equals, Air, (10.00) #sample Load Volume {base} 0.01 Block: Reset system 0.00 Base: SameAsMain 0.00 Sample flow: 0.000 {ml/min}, Off 0.00 Injection valve: Manual load 0.00 Block: Flowrate 0.00 Base: SameAsMain 0.00 System flow: (1.000)#Flow rate {ml/min}, Pre column pressure 0.00 End_Block 0.00 End_Block 0.01 End_Block 0.00 Phase: Column Wash 0.00 Base: SameAsMain (5.00)#Length of wash End_Block 0.00 Phase: Graident Elution 0.00 Base: SameAsMain 0.00 Gradient: (100.0)#Target Elution Concentration {%B}, (7.50)#Lengthof Elution {base} 10.00 End_Block 0.00 Phase: Reequilibration 0.00 Base: SameAsMain 0.00 Stop peak fractionation 0.00 Gradient: 0.0 {%B}, 0.00 {base} (7.50)#Length of reequilibration End_Block Music: "Neutrin05 - Timeless" is under a Creative Commons (CC-BY 3.0) license Music promoted by BreakingCopyright: https://bit.ly/bkc-timeless and... / @sappheiros Fragments    • Sappheiros - Fragments [Copyright & R...   Thanks for letting me use your music.