🎥 50 Basic HPLC Interview Questions | PharmaTalks 🎥 скачать в хорошем качестве

🎥 50 Basic HPLC Interview Questions | PharmaTalks 🎥

0:06 HPLC Interview Questions and Answers 0:13 What is HPLC? 0:35 What is the principle of HPLC? 1:00 What is the difference between normal-phase and reverse-phase HPLC? 1:27 What are the main components of an HPLC system? 1:53 What is a stationary phase in HPLC? 2:15 What is the mobile phase in HPLC? 2:33 What are the common detectors used in HPLC? 2:55 What is the purpose of the HPLC column? 3:15 What types of HPLC columns are available? 3:41 What is the role of the pump in HPLC? 4:01 What is the purpose of the injector in HPLC? 4:21 What is gradient elution in HPLC? 4:42 What is isocratic elution in HPLC? 5:00 How do you choose a suitable mobile phase for HPLC? 5:21 What is the purpose of the HPLC detector? 5:40 What factors affect the separation of components in HPLC? 6:01 What is retention time in HPLC? 6:18 What is peak resolution in HPLC? 6:37 What does a baseline in an HPLC chromatogram represent? 6:57 What is the purpose of a guard column in HPLC? 7:16 What is the difference between UV-Visible and PDA detectors? 7:39 What is dead time in HPLC? 7:58 What is column efficiency in HPLC? 8:16 What are theoretical plates in HPLC? 8:32 What is peak tailing in HPLC and how is it prevented? 8:54 What is peak fronting in HPLC? 9:13 What is meant by resolution in HPLC? 9:32 What is an internal standard in HPLC? 9:51 What is reverse-phase HPLC and why is it widely used? 10:13 What is HPLC gradient elution used for? 10:34 How do you optimize HPLC method development? 10:56 What is retention factor (k') in HPLC? 11:15 How is column selectivity defined? 11:34 What are some common mobile phases used in reverse-phase HPLC? 11:54 What is the purpose of buffer in HPLC mobile phases? 12:14 What is column backpressure in HPLC? 12:31 What is the purpose of degassing the mobile phase in HPLC? 12:52 How do you calculate the number of theoretical plates in HPLC? 13:15 What is system suitability in HPLC? 13:34 What are some common causes of high backpressure in HPLC? 13:55 What is the role of column temperature in HPLC? 14:14 What is the significance of peak symmetry in HPLC? 14:36 How does flow rate affect HPLC separation? 14:56 What are void volume and void time in HPLC? 15:16 What is carryover in HPLC, and how can it be minimized? 15:38 How do you clean an HPLC column? 15:59 What is peak area in HPLC, and how is it used? 16:18 What is linearity in HPLC method validation? 16:38 What is the significance of reproducibility in HPLC? 16:59 What is a calibration curve in HPLC? ......................................... Related Video    • Top 40 Analytical Method Validation Interv...      • 7 Critical Components You Must Qualify and...      • Essential Tips for #HPLC Instrument for Fr...   ......................................... Are you preparing for an interview in the field of analytical chemistry, pharmaceuticals, or biotech? This video covers the Top 50 Basic HPLC Interview Questions you need to know to ace your next interview. From understanding the principles of HPLC to method development, we’ve got all the essential topics covered! Whether you’re a beginner looking to understand High-Performance Liquid Chromatography (HPLC) or a seasoned professional polishing your knowledge, these questions and answers will help you stay on top of your game. 💼🌟 *What's Covered:*- What is HPLC? The principle of HPLC- Key components of an HPLC system Types of HPLC columns Differences between normal-phase and reverse-phase HPLC- Important HPLC terms: retention time, peak resolution, void volume, and more!🚀 Get ready to boost your interview skills and expand your understanding of one of the most important techniques in analytical chemistry! Don't forget to subscribe to our channel for more educational content on pharmaceutical guidelines, analytical techniques, and interview tips! 📚--- 📢 Subscribe to PharmaTalks for more industry-related content!