Скачать с ютуб HIV-1 RNA: HIV Infected Individuals With Viral Loads Below Limit Of Detection l Protocol Preview в хорошем качестве

HIV-1 RNA: HIV Infected Individuals With Viral Loads Below Limit Of Detection l Protocol Preview

Watch the Full Video at https://www.jove.com/v/2960/amplifyin.... Amplifying and Quantifying HIV-1 RNA in HIV Infected Individuals with Viral Loads Below the Limit of Detection by Standard Clinical Assays - a 2 minute Preview of the Experimental Protocol Helene Mens, Mary Kearney, Ann Wiegand, Jonathan Spindler, Frank Maldarelli, John W. Mellors, John M. Coffin NCI-Frederick, The virology Core at the HIV Drug Resistance Program; University of Pittsburgh, Division of Infectious Diseases; Tuffts University, Department of Molecular Biology and Microbiology; Quantifying levels of HIV-1 RNA in plasma and sequencing single HIV-1 genomes from individuals with viral loads below the limit of detection (50-75 copies/ml) is difficult. Here we describe how to extract and quantify plasma viral RNA using a real time PCR assay that reliably measures HIV-1 RNA down to 0.3 copies/ml and how to amplify viral genomes by single genome sequencing, from samples with very low viral loads. Visit https://www.jove.com?utm_source=youtube&utm_medium=social_global&utm_campaign=reseach-videos-2022 to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments