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This is an introductory video about PD-L1/CD274, intended for scientists who need to design controls and perform immunoassays detecting PD-L1/CD274. It contains information about western blot band size, protein expression, and interesting facts. Uniprot: https://www.uniprot.org/uniprot/Q9NZQ7 Human Protein Atlas: https://www.proteinatlas.org/ENSG0000... Western blot images on PMC: https://www.ncbi.nlm.nih.gov/pmc/?ter... PD-L1/CD274 Antibodies Anti-PD-L1 (CD274) Rabbit Monoclonal Antibody (M00109): https://www.bosterbio.com/anti-pd-l1-... Anti-PD-L1/CD274 Antibody Picoband™ (PB9154): https://www.bosterbio.com/anti-pd-l1-... Anti-PD-L1/Cd274 Antibody Picoband™ (A00109-3): https://www.bosterbio.com/anti-pd-l1-... Anti-PD-L1/B7-H1/CD274 Antibody Picoband™ (A00109): https://www.bosterbio.com/anti-pd-l1-... PD-L1/CD274 ELISA Kits Human PD-L1/B7-H1 ELISA Kit PicoKine™ (EK1449): https://www.bosterbio.com/human-pd-l1... Mouse PD-L1/B7-H1 ELISA Kit PicoKine™ (EK1450): https://www.bosterbio.com/mouse-pd-l1... Learn more about PD-L1/CD274 (infographic and discussion): https://www.bosterbio.com/bosterbio-g... Boster Biological Technology Website: www.bosterbio.com Email: support@bosterbio.com ________________________________________________ REFERENCES CD274. (2022). Protein Atlas. Retrieved February 17, 2022, from https://www.proteinatlas.org/ENSG0000... Dong, H., Zhu, G., Tamada, K., & Chen, L. (1999). B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nature Medicine, 5(12), 1365–1369. https://doi.org/10.1038/70932 Freeman, G. J., Long, A. J., Iwai, Y., Bourque, K., Chernova, T., Nishimura, H., Fitz, L. J., Malenkovich, N., Okazaki, T., Byrne, M. C., Horton, H. F., Fouser, L., Carter, L., Ling, V., Bowman, M. R., Carreno, B. M., Collins, M., Wood, C. R., & Honjo, T. (2000). Engagement of the Pd-1 Immunoinhibitory Receptor by a Novel B7 Family Member Leads to Negative Regulation of Lymphocyte Activation. Journal of Experimental Medicine, 192(7), 1027–1034. https://doi.org/10.1084/jem.192.7.1027 Liang, S., Latchman, Y., Buhlmann, J., Tomczak, M., Horwitz, B., Freeman, G., & Sharpe, A. (2003). Regulation of PD-1, PD-L1, and PD-L2 expression during normal and autoimmune responses. European Journal of Immunology, 33(10), 2706–2716. https://doi.org/10.1002/eji.200324228 The Nobel Prize in Physiology or Medicine 2018. (2018). The Nobel Prize. Retrieved February 17, 2022, from https://www.nobelprize.org/prizes/med... Programmed cell death 1 ligand 1. (2022). AlphaFold. Retrieved February 17, 2022, from https://alphafold.ebi.ac.uk/entry/Q9NZQ7 UniProtKB - Q9NZQ7 (PD1L1_HUMAN). (2022). Uniprot. Retrieved February 17, 2022, from https://www.uniprot.org/uniprot/Q9NZQ7