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Watch the Full Video at https://www.jove.com/v/61660/crystall.... Crystallization of Proteins on Chip by Microdialysis for In Situ X-ray Diffraction Studies - a 2 minute Preview of the Experimental Protocol Sofia Jaho, Niels Junius, Franck Borel, Yoann Sallaz-Damaz, Jean-Baptiste Salmon, Monika Budayova-Spano Université Grenoble Alpes, CEA, CNRS, IBS,; Université Bordeaux, CNRS, Solvay LOF, UMR 5258; ELVESYS SAS,; This paper details the fabrication protocol of microfluidic chips developed for on-chip protein crystallization with the dialysis method and in situ X-ray diffraction experiments. The microfabrication process makes it possible to integrate a semipermeable regenerated cellulose dialysis membrane with any molecular weight cut-off, between two layers of the chip. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments