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This on-demand roundtable webinar examines the growing shift toward animal alternatives such as stem cell based systems, organoids, organ on chip technologies, and computational models. The discussion explores why these approaches are gaining momentum, how they can improve human relevance and translational confidence, and what challenges still need to be addressed for broader adoption. The panel also dives into the ethical and regulatory forces accelerating this transition, including efforts to reduce, refine, and replace animal use in research, as well as the practical considerations researchers face when integrating new approach methodologies into existing workflows. Whether you are focused on basic discovery, translational research, or therapeutic development, this conversation offers valuable perspective on how the future of biomedical research is evolving beyond traditional animal models.