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Watch the Full Video at https://www.jove.com/v/60538/a-quanti.... A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes - a 2 minute Preview of the Experimental Protocol Rasmus Münter, Thomas Lars Andresen, Jannik Bruun Larsen Technical University of Denmark, Center for Nanomedicine and Theranostics; Technical University of Denmark, Center for Intestinal Absorption and Transport of Biopharmaceuticals; Technical University of Denmark, Department of Health Technology; This protocol describes the fabrication of liposomes and how these can be immobilized on a surface and imaged individually in a massive parallel manner using fluorescence microscopy. This allows for the quantification of the size and compositional inhomogeneity between single liposomes of the population. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments