Killing a Ciliate with light - phototoxicty (fluorescence microscopy) ΡΠΊΠ°ΡΠ°ΡΡ Π² Ρ ΠΎΡΠΎΡΠ΅ΠΌ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅
Killing a Ciliate with light - phototoxicty (fluorescence microscopy)
4 years ago
microscopy
biology
microscope
light microscope
olympus
mikroskop
fluorescence
dic
pond life
differential interference contrast microscopy
differential interference contrast
microcosmos
microphotograph
acridin orange
ciliates
ciliates under microscope
ciliate staining
microscopy staining
microscopy staining and classification
microscopy staining techniques
microscope led light source
fluorescent microscopy
fluorescent microscope
phototoxicity
phototoxic reaction
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ΠΠΎΠ²ΡΠΎΡΡΠ΅ΠΌ ΠΏΠΎΠΏΡΡΠΊΡ...
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Π‘ΠΊΠ°ΡΠ°ΡΡ Π²ΠΈΠ΄Π΅ΠΎ Ρ ΡΡΡΠ± ΠΏΠΎ ΡΡΡΠ»ΠΊΠ΅ ΠΈΠ»ΠΈ ΡΠΌΠΎΡΡΠ΅ΡΡ Π±Π΅Π· Π±Π»ΠΎΠΊΠΈΡΠΎΠ²ΠΎΠΊ Π½Π° ΡΠ°ΠΉΡΠ΅: Killing a Ciliate with light - phototoxicty (fluorescence microscopy) Π² ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅ 4k
Π£ Π½Π°Ρ Π²Ρ ΠΌΠΎΠΆΠ΅ΡΠ΅ ΠΏΠΎΡΠΌΠΎΡΡΠ΅ΡΡ Π±Π΅ΡΠΏΠ»Π°ΡΠ½ΠΎ Killing a Ciliate with light - phototoxicty (fluorescence microscopy) ΠΈΠ»ΠΈ ΡΠΊΠ°ΡΠ°ΡΡ Π² ΠΌΠ°ΠΊΡΠΈΠΌΠ°Π»ΡΠ½ΠΎΠΌ Π΄ΠΎΡΡΡΠΏΠ½ΠΎΠΌ ΠΊΠ°ΡΠ΅ΡΡΠ²Π΅, Π²ΠΈΠ΄Π΅ΠΎ ΠΊΠΎΡΠΎΡΠΎΠ΅ Π±ΡΠ»ΠΎ Π·Π°Π³ΡΡΠΆΠ΅Π½ΠΎ Π½Π° ΡΡΡΠ±. ΠΠ»Ρ Π·Π°Π³ΡΡΠ·ΠΊΠΈ Π²ΡΠ±Π΅ΡΠΈΡΠ΅ Π²Π°ΡΠΈΠ°Π½Ρ ΠΈΠ· ΡΠΎΡΠΌΡ Π½ΠΈΠΆΠ΅:
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ΠΠ½ΡΠΎΡΠΌΠ°ΡΠΈΡ ΠΏΠΎ Π·Π°Π³ΡΡΠ·ΠΊΠ΅:
Π‘ΠΊΠ°ΡΠ°ΡΡ mp3 Ρ ΡΡΡΠ±Π° ΠΎΡΠ΄Π΅Π»ΡΠ½ΡΠΌ ΡΠ°ΠΉΠ»ΠΎΠΌ. ΠΠ΅ΡΠΏΠ»Π°ΡΠ½ΡΠΉ ΡΠΈΠ½Π³ΡΠΎΠ½ Killing a Ciliate with light - phototoxicty (fluorescence microscopy) Π² ΡΠΎΡΠΌΠ°ΡΠ΅ MP3:
ΠΡΠ»ΠΈ ΠΊΠ½ΠΎΠΏΠΊΠΈ ΡΠΊΠ°ΡΠΈΠ²Π°Π½ΠΈΡ Π½Π΅
Π·Π°Π³ΡΡΠ·ΠΈΠ»ΠΈΡΡ
ΠΠΠΠΠΠ’Π ΠΠΠΠ‘Π¬ ΠΈΠ»ΠΈ ΠΎΠ±Π½ΠΎΠ²ΠΈΡΠ΅ ΡΡΡΠ°Π½ΠΈΡΡ
ΠΡΠ»ΠΈ Π²ΠΎΠ·Π½ΠΈΠΊΠ°ΡΡ ΠΏΡΠΎΠ±Π»Π΅ΠΌΡ ΡΠΎ ΡΠΊΠ°ΡΠΈΠ²Π°Π½ΠΈΠ΅ΠΌ Π²ΠΈΠ΄Π΅ΠΎ, ΠΏΠΎΠΆΠ°Π»ΡΠΉΡΡΠ° Π½Π°ΠΏΠΈΡΠΈΡΠ΅ Π² ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠΊΡ ΠΏΠΎ Π°Π΄ΡΠ΅ΡΡ Π²Π½ΠΈΠ·Ρ
ΡΡΡΠ°Π½ΠΈΡΡ.
Π‘ΠΏΠ°ΡΠΈΠ±ΠΎ Π·Π° ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ ΡΠ΅ΡΠ²ΠΈΡΠ° ClipSaver.ru
Killing a Ciliate with light - phototoxicty (fluorescence microscopy)
A short clip that shows the effect of strong excitation light on living organisms during fluorescent observations. This Ciliate was stained with acridin orange (AO) and too strong excitation light (blue light) was used. The Ciliate quickly disintegrates by loosing pieces of its membrane and it eventually explodes. The phenomenon of damaging cells during fluorescent observations is called phototoxicity. The dyes used for fluorescence microscopy can react with oxygen during excitation, which results in the formation of reactive oxygen species (ROS) which quickly react with all types of macromolecules inside of the cell, thereby damaging them. In case you also want to stain your ciliates for fluorescent observations, I have a video which explains the procedure: Β Β Β β’Β StainingΒ CiliatesΒ forΒ fluorescentΒ mic...Β Β For this demonstration, widefield fluorescence was used with an Olympus Vanox AHBT3 together with an NPL Fluotar 50/1,0 oil objective. The camera was a Panasonic S1. Excitation light was provided by a Cree XM-L2 LED.
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