Assessment Of Selective mRNA Translation In Mammalian Cells By Polysome Profiling l Protocol Preview скачать в хорошем качестве

Assessment Of Selective mRNA Translation In Mammalian Cells By Polysome Profiling l Protocol Preview

Watch the Full Video at https://www.jove.com/v/52295/assessme.... Assessment of Selective mRNA Translation in Mammalian Cells by Polysome Profiling - a 2 minute Preview of the Experimental Protocol Mame Daro Faye, Tyson E Graber, Martin Holcik University of Ottawa, Apoptosis Research Centre, Children's Hospital of Eastern Ontario Research Institute and Department of Biochemistry, Microbiology and Immunology; Montreal Neurological Institute,; University of Ottawa, Apoptosis Research Centre, Children's Hospital of Eastern Ontario Research Institute and Department of Pediatrics; The ability of cells to adapt to stress is crucial for their survival. Regulation of mRNA translation is one such adaptation strategy, providing for rapid regulation of the proteome. Here, we provide a standardized polysome profiling protocol to identify specific mRNAs that are selectively translated under stress conditions. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments