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Overview of Near-Infrared Fluorescence In near-infrared Western blot detection, proteins extracted from a biological sample are first separated on a gel by molecular weight, and then transferred to a membrane. After the empty spaces on the membrane have been blocked, a primary antibody specific for the protein of interest is added, and it binds to the target protein on the membrane. Following this step, there are two ways in which the protein of interest can be visualized and quantified using near-infrared fluorescence. Lambda U course: Preparing the Samples Lambda U Course: Preparing the Gel Lambda U Course: Transferring the Proteins About LICORbio LICORbio offers imaging systems, analysis software, and IRDye® infrared dye reagents for quantitative protein and small animal imaging. LICORbio provides an integrated solution for quantitative Western blots with its Odyssey® Infrared Imaging Systems, Image Studio™ quantification software, Empiria Studio® analysis software for publication-ready data, reagents, unique IRDye® secondary antibodies, and online training courses. For chemiluminescent Western blots, the C-DiGit® Blot Scanner is a personal, portable imager. Use the Odyssey® XF or Odyssey M Imaging Systems for both chemiluminescence and near-infrared fluorescence Western blots. You can achieve superior small animal imaging results using BrightSite™ near-infrared fluorescent probes or bioluminescence on the Pearl® Trilogy imaging system. LICORbio Website https://www.licorbio.com/ Reagent Store https://www.licor.com/bio/reagents Image Studio™ Software https://www.licor.com/image-studio LinkedIn / licorbio Twitter / licorbio Facebook / licorbio