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viva questions and answers of PS and Diff WBC count contact us - [email protected] , +916352236299 , +917863822668 Join our telegram channel - https://t.me/+RS0xSnVj00UyZTg9 #Physiology practicals #haematology #vivaquestions 0:00 Introduction 0:09 Viva questions and answers of peripheral smear and differential leucocyte count 0:23 write its function. Ans. It contains - Methylene blue - stains nuclei, and cytoplasm of WBCs and granules of basophils. Eosin - Granules of eosinophils and Hb in RBCS. Acetone free methyl alcohol - fixes smear. 1:00 Under which objective power differential count of WBC is done? Ans. oil - immersion lens. 4. Which oil is used for oil - immersion lens? Ans. cedar-wood oil. 1:18 Why one should wait for about 2 minutes after covering slide with Leishman stain? Ans. During this period, the alcohol in the stain fixes the cells of the smear called as fixation time. 6. Why one should wait for about 10 minutes after adding distilled water with Leishman stain while staining smear? Ans. During this time staining of the cells of the smear occurs called as staining time. 1:53 Which is the largest WBC in the peripheral smear? Ans. Monocyte. 8. Which is the largest cell in the bone marrow? Ans. Megakaryocyte. 9. Which is more mature lymphocyte large lymphocyte or small lymphocyte? Ans. Small lymphocyte. 2:18 How the differential counting is done in the MASTER peripheral smear? Ans. Horizontal zigzag manner from head to tail region in the peripheral smear. 11. What is the normal percentage distribution in the peripheral smear? Ans. The normal percentage distribution in the peripheral smear of 2:56 What is neutrophilia and write its causes. NEMASTER Ans. Increase in neutrophil count is known as neutrophilia It occurs in physiolocial conditions like muscular exercise, pregnancy, emotional stress, parturition, after meal. pathologically occurs in conditions like acute pyogenic infections e.g., abscess, pneumonia, after hemorrhage, steroid therapy 3:28 What is eosinophilia and write its causes.NEMASTER Ans. Increase in eosinophil count is known as eosinophilia. It occurs in various allergic conditions like bronchial asthma, food allergy, parasitic infections, various skin diseases. 3:51 What is basophilia and write its causes.KINEMASI Ans. Increase in basophil count is known as basophilia. It occurs in tuberculosis, smallpox, chronic myeloid leukemia. 15. What is lymphocytosis and write its causes. Ans. Increase in lymphocyte count is known as lymphocytosis. It occurs physiolocally in children called relative lymphocytosis. pathologically in chronic infections like tuberculosis, viral infections, chronic lymphatic leukemia. 4:36 What is monocytosis and write its causes. ASTER Ans. Increase in monocyte count is known as monocytosis. It occurs in malaria, kala azar, infectious mononucleosis. 4:53 What is neutropenia and write its causes. Ans. Decrease in neutrophil count is known as neutropenia It occurs in conditions like typhoid and paratyphoid fever, parasitic infections like malaria, viral infections like measles, aplastic anaemia, drug induced. 18. What is eosinopenia and write its causes. Ans. Decrease in eosinophil count is known as eosinopenia. It occurs in ACTH therapy, bone marrow depression. 5:38 What is basopenia and write its causes. INEMASTER Ans. Decrease in basophil count is known as basopenia. It occurs in steroid therapy, bone marrow depression. 20. What is lymphocytopenia and write its causes. Ans. Decrease in lymphocyte count is known as lymphocytopenia. It occurs AIDS, bone marrow depression. 21. What is monocytopenia and write its causes. Ans. Decrease in monocyte count is known as monocytopenia. It occurs bone marrow depression. 6:17 How the absolute count of each leucocyte is done by using value of total leucocyte count and differential leucocyte count? Ans. The formula for absolute count of each leucocyte is - differential count of the cells x total leucocyte count/100