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Introduction to Database Searching using Mascot (archive recording, 2014)

Presented by John Cottrell, Matrix Science. An overview of the three different ways to search MS and MS/MS data against sequence databases highlighting their strengths and weaknesses. This presentation can be skipped if you are already familiar with the basics of database searching. This video is part of the classic Mascot training course recorded in 2014. For up to date training material and tutorials, please check our Youtube channel and visit our website: https://www.matrixscience.com/trainin... Mascot Server product details: https://www.matrixscience.com/server.... External publications: Protein identification using MS/MS data, http://dx.doi.org/10.1016/j.jprot.201... Protein identification, the origins of peptide mass fingerprinting, http://dx.doi.org/10.1016/S1044-0305(... Computational Methods for Mass Spectrometry Proteomics, http://onlinelibrary.wiley.com/book/1... Error-tolerant identification of peptides in sequence databases by peptide sequence tags, http://dx.doi.org/10.1021/ac00096a002 An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database, http://dx.doi.org/10.1016/1044-0305(9... The interpretation of collision-induced dissociation tandem mass spectra of peptides, http://dx.doi.org/10.1002/mas.1280140104