MIT CompBio Lecture 09 - Three Dimensional Genome скачать в хорошем качестве

MIT CompBio Lecture 09 - Three Dimensional Genome

MIT Computational Biology: Genomes, Networks, Evolution, Health Prof. Manolis Kellis http://compbio.mit.edu/6.047/ Fall 2018 Lecture 09 - Three Dimensional Genome 1. Methods for studying nuclear genome organization Measuring locus-landmark interactions: ChIP, DamID Measuring locus-locus interactions: 3C, 4C, 5C, Hi-C, ChIA-PET 2. Processing and normalization of Hi-C datasets Correcting for biases and issues with Hi-C data Normalization, sources of bias and bias correction 3. Interpretation of data and functional characterization Lamina Associated Domains (LADs) Topologically Associated Domains (TADs) and other compartments 4. Conservation of nuclear genome organization Across species, across cell types Sequence basis for conservation? 5. Towards a mechanistic understanding Nuclear genome organization through the (single) cell cycle (Limitations of) 3D modeling of nuclear genome organization Loop extrusion as a potential mechanism for nuclear organization Slides for Lecture 9: https://stellar.mit.edu/S/course/6/fa...