Anthony Hall. A Genome-Wide Survey of DNA Methylation in Hexaploid Wheat скачать в хорошем качестве

Anthony Hall. A Genome-Wide Survey of DNA Methylation in Hexaploid Wheat

DNA methylation is an important mechanism of epigenetic gene expression control that can be passed between generations. Here, we use sodium bisulphite treatment and targeted gene enrichment to study genome-wide methylation across the three sub-genomes of allohexaploid wheat. While the majority of methylation is conserved across all three genomes we demonstrate that differential methylation exists between the sub-genomes in approximately equal proportions. We correlate sub-genome specific promoter methylation with decreased expression levels and show that altered growing temperature has a small effect on methylation state, identifying a small but functionally relevant set of methylated genes. Finally, we demonstrate long-term methylation maintenance using a comparison between the D sub-genome of hexaploid wheat and its progenitor Aegilops tauschii. I will also describe how we are using this technology to investigate the role of epigenetics in the stabilisation of a new polyploid.