Detection of Plant Hormones by Ion Mobility Spectrometry | Vahideh Ilbeigi скачать в хорошем качестве

Detection of Plant Hormones by Ion Mobility Spectrometry | Vahideh Ilbeigi

Ion mobility spectrometry (IMS) is an analytical technique for the gas phase detection of ions based on their mobilities. We are working on the development of IMS-based methods for the measurement of analytes in plant and food samples. Plant hormones (PHs) are signalling molecules produced within plants that influence plant growth, seed germination, fruit maturation, and ripening. Hence, the quantitative analysis of the PHs and determination of their concentrations in different tissues is crucially important in understanding the role of these molecules in physiological processes occurring in plants. We developed an IMS-based method to measure methyl salicylate (MeSA), one of the important plant hormones, in tomato leaves. For this purpose, we utilized solid phase microextraction (SPME) to extract MeSA, followed by a pre-separation using a multicapillary column (MCC) before analyzing the sample with IMS. We have also developed an IMS-based method for the in-vivo detection of the volatile organic compounds (VOCs) emitted from the blueberry plant. A glass chamber equipped with a Tenax absorbent column was designed and constructed to collect the emitted VOCs from the blueberry plant. The complex VOCs matrix was separated using a combination of an MCC column and IMS. The developed Tenax-MCC-IMS method was used for the detection of the emitted VOCs from blueberry leaves upon exposure to different stress factors including mechanical (punching), herbivore (aphids) and methyl jasmonate (MeJA) spraying. .. #math #science #physics #informatics #comeniusuniversity #slovakia #lecture #sciencelover #sciencelecture