RNA Polymerase II Elongation Complex for cutting RNA Co-transcription | Protocol Preview скачать в хорошем качестве

RNA Polymerase II Elongation Complex for cutting RNA Co-transcription | Protocol Preview

Watch the Full Video at https://www.jove.com/v/59497/artifici.... Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events - a 2 minute Preview of the Experimental Protocol Melvin Noe Gonzalez, Joan W. Conaway, Ronald C. Conaway Stowers Institute for Medical Research,; Kansas University Medical Center, Department of Biochemistry and Molecular Biology; Here, we describe the assembly of RNA polymerase II (Pol II) elongation complexes requiring only short synthetic DNA and RNA oligonucleotides and purified Pol II. These complexes are useful for studying mechanisms underlying co-transcriptional processing of transcripts associated with the Pol II elongation complex. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel:    / jovejournalofvisualizedexperiments