Beam Splitters and the Advanced Filter Block (plus Microscope Length and Distance RMS/DIN Standards) скачать в хорошем качестве

Beam Splitters and the Advanced Filter Block (plus Microscope Length and Distance RMS/DIN Standards)

PUMA is a DIY open source portable 3D printed microscope with augmented reality, fluorescence, polarisation, dark ground, phase contrast, epi-illumination and other advanced features. Beam splitters are essential for more advanced light microscopy techniques such as co-axial epi-illumination and binocular heads. For PUMA a splitter is also used as a beam joiner for the Augmented Reality Heads Up Display (AR HUD). This video includes a tutorial section about beam splitters and also illustrates several RMS / DIN standard lengths and distances used in microscopy such as the mechanical tube length and par-focal distance, etc. At the end I show you how to build the two beam splitter components used in PUMA - the advanced filter block and the splitter component of the binocular head. CONTENTS ======== 00:08 What this talk will cover 00:40 Microscope RMS / DIN standards 05:42 Introduction to infinity-corrected systems 07:07 Standard lengths illustrated on a PUMA scope 08:49 Introduction to the PUMA filter blocks 09:12 Introduction to PUMA monocular and binocular heads 09:52 Support the Open Source PUMA Project 10:14 Beam splitters and optical aberrations 13:53 Demonstration of beam paths in the PUMA beam splitters 18:49 How to build the beam splitter of the binocular head module 27:49 How to build the advanced filter block 45:14 Conclusion SUPPORT PUMA ============ PUMA is an open source microscopy project. You can help support the project by: 1. Become a Patron:   / pumamicroscope   2. Donate via PayPal: https://www.paypal.com/donate/?hosted... 3. Simply subscribing to this YouTube channel, like, comment and share these videos. 4. Spread the word - post the news about PUMA and link to this YouTube channel on your social media sites and any other outreach method (tell your friends, colleagues, students or teachers and lecturers about PUMA, for example). 5. Consider purchasing your optics and related supplies from our affiliated online optics store, OptArc.co.uk ( https://www.optarc.co.uk/ ) PARTS AND TOOLS =============== General ------- For more details see the 'Bill_of_Materials' on the PUMA GitHub page ( https://github.com/TadPath/PUMA ) which also gives examples of where you can buy some of the more specialised items. NOTE: Some models may be updated since the v.1 release of PUMA. Always print the latest models from the main branch of the GitHub page. If you printed models in advance of the release date of this video you may need to re-print some as detailed below. Tools ----- Allen keys for the bolts Print clean-up tools (e.g. craft knife) 2 cm diameter separator tool (I use a 1p coin - see video) 3D Printed Models (Binocular Head Splitter) ------------------------------------------- BN_Ocular_Base_thread_L.stl or BN_Ocular_Base_thread_R.stl BN_Outlet_upper.stl (REVISED SINCE V1 - PRINT THE LATEST ONE) BN_Splitter_block.stl (REVISED SINCE V1 - PRINT THE LATEST ONE) DI_Epi_cap.stl (1) Non-Printed Parts ----------------- M3_Allen_Bolt_Socket_Cap_DIN912_12mm_long (6) 50:50 Beamsplitter_glass_2cm_square_1.1mm_thick (1) Mirror_2cm_square_first_surface_full_2mm_thick (1) 3D Printed Models (Advanced Filter Block) ----------------------------------------- FB_Filter_slot_top.stl FB_Filter_slot_bottom.stl (2) FB_Infinity_adapter.stl FB_Side_port_separate.stl FB_Splitter_case.stl FB_Stopper.stl (3) DI_Epi_cap.stl (1) FB_Top_connector.stl FB_Filter_slider.stl (3 or as many as you need) FB_Filter_collar.stl (3 or as many as you need) QR_Base_thread.stl QR_C-RMS_Thread.stl QR_Male_C_extn_1mm.stl Non-Printed Parts ----------------- M2_Allen_Bolt_Socket_Cap_DIN912_10mm_long for the Infinity adapter (4) M2_Allen_Bolt_Socket_Cap_DIN912_12mm_long for the AR HUD port and top connector (6) M3_Grub_Screw_Cup_point_Hex_socket_DIN916_5mm_long for the QR objective holder (3) M3_Allen_Bolt_Socket_Cap_DIN912_14mm_long to connect the base to the splitter case (4) M3_Full_Nut (1) 50:50 Beamsplitter_glass_2cm_square_1.1mm_thick (1) --- FOR FURTHER INFORMATION ======================= 1. Open access write-up about PUMA on the FreeCAD blog: https://blog.freecad.org/2023/02/13/a... 2. The official PUMA GitHub page where you can download the specs and source files to build or customise your own PUMA system: https://github.com/TadPath/PUMA 3. The scientific peer reviewed publication on PUMA that was published in 2021 in the Journal of Microscopy (a journal of the Royal Microscopical Society) available here: https://pubmed.ncbi.nlm.nih.gov/34151... https://onlinelibrary.wiley.com/doi/1... Thanks for your interest in the PUMA microscope system. PJT 20/01/22