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Watch the Full Video at https://www.jove.com/v/60034/perturbi.... Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption - a 2 minute Preview of the Experimental Protocol Md. Mydul Islam, Robert L. Steward, Jr. University of Central Florida, Department of Mechanical and Aerospace Engineering; University of Central Florida, Burnett School of Biomedical Sciences, College of Medicine; Here, we present a mechanics-based protocol to disrupt the gap junction connexin 43 and measure the subsequent impact this has on endothelial biomechanics via observation of tractions and intercellular stresses. Visit https://www.jove.com?utm_source=youtu... to explore our entire library of 14,000+ videos of laboratory methods and science concepts. JoVE is the world-leading producer and provider of science videos with the mission to improve scientific research and education. Millions of scientists, educators, and students at 1500+ institutions worldwide, including schools like Harvard, MIT and Stanford benefit from using JoVE's extensive library of 14,000+ videos in their research,education and teaching. Subscribe to our channel: / jovejournalofvisualizedexperiments